Fig. 1
From: PCR-based reverse genetics strategy for bluetongue virus recovery
Schematic representation for PCR amplicon. Stably expressing T7 polymerase, and DNA with T7 polymerase promoter could be transcribed into RNA in the BSR cell. To prepare the template for RNA transcription to rescue BTV with RG system, the T7 polymerase promoter was introduced into the 5′ terminal of each gene by PCR method. The first nucleotide of BTV segments was located at transcriptional start site of T7 polymerase and the last nucleotide of PCR amplicon was the last one of BTV segments. This ensures the RNA synthesized with theses templates in the BSR are exactly same as corresponding original segments of BTV