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Fig. 6 | Virology Journal

Fig. 6

From: Histone deacetylase 1 interacts with HIV-1 Integrase and modulates viral replication

Fig. 6

HDAC1 overexpression affects HIV-1 expression. a, b HeLa-CD4-LTR-β-gal (a) or 293 T (b) cells were transfected with 20 nM siHDAC1 (Hs_HDAC1_6) or control siRNA (GFP-22 siRNA) and, after 48 h, transfected with pNL4–3.Luc.R-E- (1.6 μg/105 cells). HDAC1 knockdown was determined by Western blot analysis using anti-HDAC1 and anti-β-actin antibodies (lower panels). Luciferase activity was measured in cell lysates 24 h after proviral DNA transfection and normalized for protein levels. c 293 T cells were transfected with pFLAG-HDAC1 or the pBact-FLAG empty vector (375 ng per 105 cells) and 48 h later, transfected with pNL4–3.Luc.R-E-. Luciferase activity was measured in cell lysates 24 h after proviral DNA transfection and normalized for protein levels. Cell lysates were analyzed by Western blot using anti-FLAG and anti-β-actin antibodies (right panel). Results derived from the measurement of luciferase activities were calculated as means ± SD of six measurements from a single experiment (n = 6) and are representative of two to four independent experiments. Results were analysed by the Student t test (***, p < 0.001)

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