Table 1 Primers used in this study
Primer | Sequence (5′–3′) | Use |
|---|---|---|
C-F | GTACTCGAGATGTCTGATGATGGCGCA | Amplification of C, CΔC, CΔ8, CΔ7, CΔ5 and CΔ2 |
CΔN-F | TACTCGAGATGCCACCCGACGCT | Amplification of CΔN |
C-R | ATGGATCCCGGGCTTCAACTGGTT | Amplification of C and CΔN |
CΔC-R | ATGGATCCCGCTTAGTTCTGCTGTC | Amplification of CΔC |
CΔ8-R | CGGATCCCGAATTGTTATTACCGC | Amplification of CΔ8 |
CΔ7-R | CGGATCCCGCATAATTGTTATTAC | Amplification of CΔ7 |
CΔ5-R | CGGATCCCGATACAACATAATTGT | Amplification of CΔ5 |
CΔ2-R | CGGATCCCGAACTGGTTGATACAA | Amplification of CΔ2 |
qPCR-EGFP-F | ATGGCCGACAAGCAGAAGAA | qRT-PCR for detection of EGFP |
qPCR-EGFP-R | AACTCCAGCAGGACCATGTG | |
qPCR-C-F | ACAGCAGAACTAAGCCACCC | qRT-PCR for detection of C |
qPCR-C-R | TCTTGTTGTGGTACAGGCCG | |
qPCR-GAPDH-F | GAAGGTCGGAGTGAACGGATTT | qRT-PCR for detection of GAPDH |
qPCR-GAPDH-R | TGGGTGGAATCATACTGGAACA |