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Table 3 The DNA fragments used for construction of GPV RC16 infectious clone with mutated NLS site in this study

From: The 164 K, 165 K, and 167 K residues of VP1 are vital for goose parvovirus proliferation in GEFs based on PCR-based reverse genetics system

DNA fragments

The sequence (5′-3′)

K164A

GCAAAAAAAAATACAGGGAAGCTTACCGACCACTACCCGGTAGTTGCAAAGCCTAAACTTACCGAGGAAGTCAGTGCGGGAGGTGGTAGTAGTGCCGTACAAGACGGAGGAGCCACCGCGGAGGGTACCGAACCTGTGGCAGCAT

K165A

GCAAAAAAAAATACAGGGAAGCTTACCGACCACTACCCGGTAGTTAAGGCACCTAAACTTACCGAGGAAGTCAGTGCGGGAGGTGGTAGTAGTGCCGTACAAGACGGAGGAGCCACCGCGGAGGGTACCGAACCTGTGGCAGCATC

K167A

GCAAAAAAAAATACAGGGAAGCTTACCGACCACTACCCGGTAGTTAAGAAGCCTGCACTTACCGAGGAAGTCAGTGCGGGAGGTGGTAGTAGTGCCGTACAAGACGGAGGAGCCACCGCGGAGGGTACCGAACCTGTGGCAGCATC

AAPA

GCAAAAAAAAATACAGGGAAGCTTACCGACCACTACCCGGTAGTTGCAGCACCTGCACTTACCGAGGAAGTCAGTGCGGGAGGTGGTAGTAGTGCCGTACAAGACGGAGGAGCCACCGCGGAGGGTACCGAACCTGTGGCAGCATC

KKRK

GCAAAAAAAAATACAGGGAAGCTTACCGACCACTACCCGGTAGTTAAGAAGAGGAAACTTACCGAGGAAGTCAGTGCGGGAGGTGGTAGTAGTGCCGTACAAGACGGAGGAGCCACCGCGGAGGGTACCGAACCTGTGGCAGCATC

  1. The mutated sites were indicated by underline
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Virology Journal

ISSN: 1743-422X