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Fig. 2 | Virology Journal

Fig. 2

From: The 164 K, 165 K, and 167 K residues of VP1 are vital for goose parvovirus proliferation in GEFs based on PCR-based reverse genetics system

Fig. 2

The construction of GPV RC16 reverse genetics strategy. a Six pair primers had been designed to amplify the complete genome based on the genome of the GPV YZ99–6 strain. The amplified fragments were A1, A2, A3, A4, A5, and A6, respectively. All fragments from A1 to A6 were orderly cloned into pACYC177. A genetic maker (M: AAGCTT→GAGTTT) was introduced into the site 1265 and 1268. b The amplified six fragments have been identified by Electrophoresis

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Virology Journal

ISSN: 1743-422X