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Table 3 Results of consistency evaluation of MPBT assay (titers are expressed on log10CCID50/ml)

From: Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Sabin strain

Repeat codes

Mean

SD

RSD %

1

2

3

4

5

Sabin 1a

8.75

8.63

9.10

8.75

8.75

8.80

0.18

42.69

Sabin 1b

8.66

8.77

8.84

9.07

8.51

8.77

0.21

51.03

Sabin 2a

8.63

8.63

8.75

9.25

8.75

8.80

0.26

64.91

Sabin 2b

8.28

8.39

8.51

8.51

8.28

8.39

0.12

26.96

Sabin 3a

8.88

8.75

8.88

9.00

9.25

8.95

0.19

45.56

Sabin b

8.32

8.13

8.43

8.24

8.35

8.29

0.11

26.74

  1. SD Standard deviation of log10 titers, RSD Relative standard deviation
  2. aVirus was titrated with 10-fold dilution
  3. bvirus was titrated with 2-fold dilution
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Virology Journal

ISSN: 1743-422X