Fig. 2

Phylogenetic trees for CrTVs and other members in the family Totiviridae based on the deduced amino acid sequences for the RdRp (a) and CP (b). Sequences were aligned using Clustal Omega and trees were generated using the maximum likelihood method with a bootstrap test of 100 iterations in MEGA 6 software. Five approved genera are labelled for the well-supported clades on each tree. Bootstrap support resulting from 100 replicates is shown on the internodes and branch lengths correspond to genetic distance; the scale bar at lower left corresponds to a genetic distance of 0.5 for the protein sequences. Virus sequences were downloaded from GenBank and presented here as: Anopheles totivirus (AnTV, KX148550), Armigeres subalbatus virus (AsTV, EU715328), Aspergillus foetidus slow virus 1 (AFSV-1, HE588147), Beihai barnacle virus 15 (BBV15, NC_032413), Black raspberry virus F (BRV-F, NC_009890), Camponotus nipponicus virus (CNV, NC_029312), Camponotus yamaokai virus (CYV, NC_027212), Diatom colony associated dsRNA virus 17 (DcaRV-17A, BAU79516; DcaRV-17B, BAU79518), Drosophila melanogaster totivirus (DTV, GQ342961), Eimeria brunetti RNA virus 1 (EbRV1, AF356189), Eimeria stiedai RNA virus 1 (EsRV-1, KU597305), Eimeria tenella RNA virus 1 (EtRV1, NC_026140), Giardia canis virus (GcV, DQ238861), Giardia lamblia virus (GlV, NC_003555), Gremmeniella abietina RNA virus L1 (GARV-L1, NC_003876), Helicobasidium mompa totivirus 1–17(HMV1–17, NC_005074), Helminthosporium victoriae virus 190S (HVV-190S, NC_003607), Hubei toti-like virus 6 (HTLV6, KX882940), Leishmania RNA virus 1 (LRV1–1, M92355), Leishmania RNA virus 2 (LRV2–1, U32108), Magnaporthe oryzae virus 1 (MOV-1, NC_006367), Maize-associated totivirus 1 (MaTV-1, NC_028044), Omono iiver virus (ORV, AB555544), Panax notoginseng virus A (PnV-A, NC_029096), Penaeid shrimp infectious myonecrosis virus (IMNV, AY570982), Persimmon latent virus (PeLV, NC_023983); Phakopsora pachyrhizi mycovirus (PaMV, KT222801), Piscine myocarditis-like virus (PMCLV, NC_029302), Puccinia striiformis totivirus (PsV1, KY207361; PsV2, KY207362; PsV3, KY207363; PsV5, KY207365), Pythium polare RNA virus 2 (PpRV2, LC376045), Red clover powdery mildew-associated totivirus (RPaTV1a, NC_028480;RPaTV2, NC_028481; RPaTV3, NC_028483; RPaTV4, LC075489; RPaTV5, NC_028485; RPaTV6, NC_028486; RPaTV7, NC_028488; RPaTV8, LC075493; RPaTV9, NC_028490), Rosellinia necatrix victorivirus 1 (RNV-1, NC_021565), Saccharomyces cerevisiae virus (SCV-L-BC, U01060; SCV-LA, NC_003745), Scheffersomyces segobiensis virus L (SSV-L, KC610514), Sogatella furcifera virus (SfTV1, MG546515; SfTV2, MG546516), Tolypocladium cylindrosporum virus 1 (TCV1, NC_014823), Trichomonas vaginalis virus (TVV1, TVU08999;TVV2, AF127178;TVV3, AF325840;TVV4, HQ607522), Tuber aestivum virus 1 (TAV1, HQ158596), Ustilago maydis virus H1 (UmV-H1, NC_003823), and Xanthophyllomyces dendrorhous virus (XDV-L1A, JN997472; XDV-L1B, JN997473)