Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: Characterization of Cronartium ribicola dsRNAs reveals novel members of the family Totiviridae and viral association with fungal virulence

Fig. 1

Analysis of the full-length genomes of CrTVs. a Agarose gel electrophoresis of dsRNA extracted from Cronartium ribicola spores and cankered stems of western white pine. Two cankered western white pine stem samples (lane 1–2) shows presence of dsRNA with genomic DNA and partially degraded rRNAs without enzyme treatment. Three of four spore samples (lane 3: DNA standard ladder; lane 4–5: urediniospores; lane 6–7: aeciospores) show obvious presence of dsRNA after treatment with DNase I, nuclease S1. b Diagrammatic representation of CrTV genome organization. Open reading frame (ORF) positions, reading phases, and putative protein lengths are labelled. ORF1 and ORF2 encode putative capsid protein and RdRp respectively; and they overlap in four genomes CrTV1, CrTV2, CrTV3 and CrTV4. ORF2 is presumptively translated as a fusion protein with ORF1 through a + 1 ribosomal frameshift for CrTV1, or through a − 1 ribosomal frameshift for CrTV2, CrTV3, and CrTV4. c Putative pseudoknots were predicted using the HPknotter program and viewed using the PseudoViewer3 program. Spacer distances were calculated from the pseudoknot upstream to the potential slippery site for CrTV2, CrTV3, and CrTV4, or upstream to the in-frame stop codon of ORF2 for CrTV1. Minimal free energy (MEF) are presented

Back to article page