Fig. 2

Nsp3 protein in TGEV plays a crucial role in the inhibition of NF-κB signaling pathway. a IPEC-J2 cells and HEK-293 T cells were co-transfected with pNF-κB-Luc (0.5 μg), pRL-TK (0.025 μg), and the respective expression plasmid encoding either the TGEV protein or truncated segments (0.5 μg). At 24 h post-transfection, the cells were treated with poly (I:C). The cell lysates were prepared at 12 h post-treatment and subjected to luciferase activity assay. b Increasing doses of Nsp3-expressing plasmids (0, 0.5, 1.0, and 1.5 μg), pNF-κB-Luc (0.5 μg), and pRL-TK (0.025 μg) were co-transfected into the IPEC-J2 cells and HEK-293 T cells. At 24 h post-transfection, poly(I:C) was added to activate the NF-κB signaling pathway. The cell samples were collected at 36 h post-transfection and subjected to luciferase activity assay. Results are representative of three independent experiments. Data are presented as mean ± SD. *P < 0.05 and **P < 0.01 were considered to be statistically significant and highly significant, respectively