Fig. 1
From: The effects of autophagy on the replication of Nelson Bay orthoreovirus
Production of NBV-MB by reverse genetics, confirmed by IFA and CPE. a BHK cells were transfected with viral plasmids and pcDNA3.1-T7. After 7 days, the viruses were collected and used to infect BHK cells, which were subjected to IFA after 24 h. b Viral infection induced CPE in BHK cells. BHK cells were infected with NBV-MB viruses recovered from the reverse genetics system; after 48 h, CPE was observed under a microscope