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Table 1 The genome characteristics of melon chlorotic spot virus (MeCSV) isolate (JKI 29327) from Austria; nt and aa sequence identities of the 8 RNA segments compared to the respective homologous regions in the genome of MeCSV isolate E11–018 from France and the list of primers used for segment identification

From: A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria

Genome segments Intergenic region (IR) Predicted proteins
Type Length (nt) Percent nt identity to MeCSV E11–018 PCR primers used for JKI 29327 Length (nt) Percent nt identity to MeCSV E11–018 ORF Putative functions Length (aa) Size (kDa) Percent aa identity to MeCSV E11–018
Name Sequence
RNA1 9092 82.4 HZ-603 5′ ACA GAA GTG GAA TGG GCT GG 3’ NA NA ORF1 RNA-dependent RNA polymerase 2940 340 92.1
HZ-604 5′ GCA ACA CCC TCA TCA CTC CA 3’
RNA2 1847 84.6 HZ-605 5′ AGC TCA GTA ACC GGA ACT GC 3’ 50 100 ORF2a no match 373 43.6 87.7
HZ-606 5′ CGC AAT AGC AGG GTC CAG AT 3’ ORF2b no match 196 43.7 87.3
RNA3 1598 85.8 HZ-607 5′ TGG TGC CAG AAG GAA AGG AC 3’ 157 72.6 ORF3a no match 195 23.2 93.3
HZ-608 5′ GGC AAT GCC TCA CAA TCG TC 3’ ORF3b no match 235 27.6 89.8
RNA4 1591 78.3 HZ-609 5′ AAG TAA GGG CAG GCT GAA CC 3’ NA NA ORF4 nucleocapsid protein 305 33.6 88.2
HZ-610 5′ AGG CTT TCT GCT AAG TGG GC 3’
RNA5 1547 81.7 HZ-611 5′ GAA CTG TAC CGC TGA TGG GT 3’ 446 68.3 ORF5a no match 210 23.2 93.3
HZ-612 5′ CTT TGG TCT GGA GCT GTG CT 3’ ORF5b no match 114 13 95.6
RNA6 1509 84.7 HZ-613 5′ CGC ATC CTG AAT CCC ATC TCT 3’ NA NA ORF6 no match 400 45.5 91.2
HZ-614 5′ GCT GGC ATC ACT AGA CGG AT 3’
RNA7 1392 73.2 HZ-615 5′ ATC AGG TGT TAG CTG GCC AC 3’ 586 55.6 ORF7a no match 84 9.8 90.5
HZ-616 5′ TAA CCA CCT TCC CTG CTG TG 3’ ORF7b no match 143 15.9 80.6
RNA8 1229 68.7 HZ-617 5′ ACC CTA AGT GGA TCC GAG GT 3’ 446 58.3 ORF8a no match 93 10.9 94.8
HZ-618 5′ AGT TCC AAG TTG CCC TGC TT 3’ ORF8b no match 115 12.9 97.2
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Virology Journal

ISSN: 1743-422X