A divergent strain of melon chlorotic spot virus isolated from black medic ( Medicago lupulina ) in Austria

Yahya Z. A. Gaafar; Katja R. Richert-Pöggeler; Angelika Sieg-Müller; Petra Lüddecke; Kerstin Herz; Jonas Hartrick; Yvonne Seide; Heinrich-Josef Vetten; Heiko Ziebell

doi:10.1186/s12985-019-1195-8

Virology Journal

Table 1 The genome characteristics of melon chlorotic spot virus (MeCSV) isolate (JKI 29327) from Austria; nt and aa sequence identities of the 8 RNA segments compared to the respective homologous regions in the genome of MeCSV isolate E11–018 from France and the list of primers used for segment identification

From: A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria

Genome segments					Intergenic region (IR)		Predicted proteins
Type	Length (nt)	Percent nt identity to MeCSV E11–018	PCR primers used for JKI 29327		Length (nt)	Percent nt identity to MeCSV E11–018	ORF	Putative functions	Length (aa)	Size (kDa)	Percent aa identity to MeCSV E11–018
Type	Length (nt)	Percent nt identity to MeCSV E11–018	Name	Sequence	Length (nt)	Percent nt identity to MeCSV E11–018	ORF	Putative functions	Length (aa)	Size (kDa)	Percent aa identity to MeCSV E11–018
RNA1	9092	82.4	HZ-603	5′ ACA GAA GTG GAA TGG GCT GG 3’	NA	NA	ORF1	RNA-dependent RNA polymerase	2940	340	92.1
RNA1	9092	82.4	HZ-604	5′ GCA ACA CCC TCA TCA CTC CA 3’	NA	NA	ORF1	RNA-dependent RNA polymerase	2940	340	92.1
RNA2	1847	84.6	HZ-605	5′ AGC TCA GTA ACC GGA ACT GC 3’	50	100	ORF2a	no match	373	43.6	87.7
RNA2	1847	84.6	HZ-606	5′ CGC AAT AGC AGG GTC CAG AT 3’	50	100	ORF2b	no match	196	43.7	87.3
RNA3	1598	85.8	HZ-607	5′ TGG TGC CAG AAG GAA AGG AC 3’	157	72.6	ORF3a	no match	195	23.2	93.3
RNA3	1598	85.8	HZ-608	5′ GGC AAT GCC TCA CAA TCG TC 3’	157	72.6	ORF3b	no match	235	27.6	89.8
RNA4	1591	78.3	HZ-609	5′ AAG TAA GGG CAG GCT GAA CC 3’	NA	NA	ORF4	nucleocapsid protein	305	33.6	88.2
RNA4	1591	78.3	HZ-610	5′ AGG CTT TCT GCT AAG TGG GC 3’	NA	NA	ORF4	nucleocapsid protein	305	33.6	88.2
RNA5	1547	81.7	HZ-611	5′ GAA CTG TAC CGC TGA TGG GT 3’	446	68.3	ORF5a	no match	210	23.2	93.3
RNA5	1547	81.7	HZ-612	5′ CTT TGG TCT GGA GCT GTG CT 3’	446	68.3	ORF5b	no match	114	13	95.6
RNA6	1509	84.7	HZ-613	5′ CGC ATC CTG AAT CCC ATC TCT 3’	NA	NA	ORF6	no match	400	45.5	91.2
RNA6	1509	84.7	HZ-614	5′ GCT GGC ATC ACT AGA CGG AT 3’	NA	NA	ORF6	no match	400	45.5	91.2
RNA7	1392	73.2	HZ-615	5′ ATC AGG TGT TAG CTG GCC AC 3’	586	55.6	ORF7a	no match	84	9.8	90.5
RNA7	1392	73.2	HZ-616	5′ TAA CCA CCT TCC CTG CTG TG 3’	586	55.6	ORF7b	no match	143	15.9	80.6
RNA8	1229	68.7	HZ-617	5′ ACC CTA AGT GGA TCC GAG GT 3’	446	58.3	ORF8a	no match	93	10.9	94.8
RNA8	1229	68.7	HZ-618	5′ AGT TCC AAG TTG CCC TGC TT 3’	446	58.3	ORF8b	no match	115	12.9	97.2

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