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Fig. 4 | Virology Journal

Fig. 4

From: A GFP-tagged version of the pseudorabies virus protein UL56 localizes to the Golgi and trans-Golgi network through a predicted C-terminal leucine-rich helix in transfected cells

Fig. 4

Subcellular distribution of pUL56 truncations in transfected Vero cells. a Schematic representation of pUL56 fused with GFP at the N-terminus. Five functional domains/motifs including hydrophobic region (48–66, 108–121, 147–156, and 174–204 aa), PPxY motifs (74–77, 101–104, 112–115, and 122–125 aa), PxxP motifs (18–21, 91–94, 120–123, and 132–137 aa) and one predicted transmembrane helix (177–203 aa) are labelled with different colors. b-d Representative confocal micrographs show subcellular distribution of pUL56 truncations in transfected Vero cells. e Western blot shows the molecular weight of GFP fused WT pUL56 and its truncations. Scale bar indicates 5 μm (b-d)

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