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Fig. 2 | Virology Journal

Fig. 2

From: Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7

Fig. 2

A1: Amplification curves 1 to 5 indicate a dilution range from 104 to 100 copies/reaction of HAdV 3 standard plasmid with 1000 copies of the IC in the HEX (5-hexachlorofuorescein) detection channel. A2: Amplification curves for 1000 copies of the IC combined with (curves 1 to 5) 104 to 100 copies/reaction of HAdV 3 standard plasmid, in the FAM (6-carboxyfuorescein) detection channel. The same color amplification curves in A1 and A2 indicate use of the same pair of primers in the reaction tube. B1: Amplification curves 1 to 4 indicate a dilution range from 104 to 10 copies/reaction of HAdV 7 standard plasmid with 1000 copies of the IC in the HEX detection channel. B2: Amplification curves for 1000 copies of the IC combined with (curves 1 to 4) 104 to 10 copies/reaction of HAdV 7 standard plasmid, in the FAM detection channel. The same color amplification curves in B1 and B2 indicate use of the same pair of primers in the reaction tube

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