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Fig. 2 | Virology Journal

Fig. 2

From: Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens

Fig. 2

Intensity of the colorimetric output of the antibody-bound alkaline phosphatase reactions (panels a (NC), c (N), e (V), g (P) and i (P-18)) and a structural representation of each mapped region on the crystal structure of VP2 for each condition (panels b (NC), d (N), f (V), h (P) and j (P-18)). The signal intensity of the positive spots is shown in Fig. 1c, based on sample collection days D0, D28, D58 and D72, and was used to reconstruct epitope maps of PPV in graphs for the whole protein. The X-axis corresponds to the spot position (in agreement with Fig. 1) and four results are shown per spot. The Y-axis shows the relative intensity for each spot. The data was normalized for each experimental condition and a threshold was set at 40% of the maximum intensity. The models on the right show the crystallographic structure of VP2 protein (PDB entry 1k3v) with positivity indicated as yellow for D28 and/or D58, red for D72, and orange for positivity in both D28 and/or D58 and D72

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Virology Journal

ISSN: 1743-422X