Table 2 Primers used in this study
From: Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization
Primer | Sequence (5′-3′) |
|---|---|
pET32a-3C-F | CATATGATGCACCATCATCATCATCATAGCGGGCGGGTGAATTTCAGACATA |
pET32a-3C-R | AAGCTTTTATTGATTAAAAACTGGAAAGACCCTA |
3C-H48A-R | GTAAATTTAGACGCCCCAAATGTCA |
3C-H48A-F | TTTGGGGCGTCTAAATTTACACAAT |
3C-C144A-R | CAAGCACACCACCCGCGGAGCCAGG |
3C-C144A-F | GGCTCCGCGGGTGGTGTGCTTGTAG |
EGFP-3C-F | GAATTCTTATGAGCGGGCGGGTGAATTTCAGACATA |
EGFP-3C-R | GGATCCGGTTGATTAAAAACTGGAAAGACCCTA |