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Table 5 Replication experiment to evaluate precision (within-run and between-run imprecision) of the multiplex RT-qPCR assays for the detection and genotyping of equine rotavirus A

From: Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

Concentration of target (IVT RNA copies/μl) Within-run imprecision Between-run imprecision
G3 G14 NSP3 G3 G14 NSP3
100,000 2.63% 1.29% 0.89% 2.92% 1.56% 0.97%
10,000 1.75% 0.74% 0.42% 2.19% 1.24% 0.83%
1,000 2.01% 0.51% 0.56% 2.52% 0.63% 0.68%