Table 5 Replication experiment to evaluate precision (within-run and between-run imprecision) of the multiplex RT-qPCR assays for the detection and genotyping of equine rotavirus A
Concentration of target (IVT RNA copies/μl) | Within-run imprecision | Between-run imprecision | ||||
|---|---|---|---|---|---|---|
G3 | G14 | NSP3 | G3 | G14 | NSP3 | |
100,000 | 2.63% | 1.29% | 0.89% | 2.92% | 1.56% | 0.97% |
10,000 | 1.75% | 0.74% | 0.42% | 2.19% | 1.24% | 0.83% |
1,000 | 2.01% | 0.51% | 0.56% | 2.52% | 0.63% | 0.68% |