Table 4 Analytical sensitivity analysis of singleplex and multiplex RT-qPCR assays for the detection and genotyping of equine rotavirus A
Parameter | Singleplex | Multiplex | ||||
|---|---|---|---|---|---|---|
G3 | G14 | NSP3 | G3 | G14 | NSP3 | |
Slope | −3.3936 | −3.3732 | − 3.2533 | −3.4104 | − 3.6377 | −3.3175 |
Linearity (R2) | > 0.99 | > 0.99 | > 0.99 | > 0.99 | > 0.99 | > 0.99 |
Efficiency (%) | 97 | 98 | 103 | 96.4 | 88 | 100 |
LOD95% (copies/μl) | 2.6 | 5.7 | 27 | 716 | 215 | 47 |
Detection rate limit (100%, copies/μl) | 10 | 10 | 100 | 1000 | 1000 | 100 |
Ct cut-off | 38 | 39 | 34 | 32 | 34 | 34 |