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Table 4 Analytical sensitivity analysis of singleplex and multiplex RT-qPCR assays for the detection and genotyping of equine rotavirus A

From: Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

Parameter Singleplex Multiplex
G3 G14 NSP3 G3 G14 NSP3
Slope −3.3936 −3.3732 − 3.2533 −3.4104 − 3.6377 −3.3175
Linearity (R2) > 0.99 > 0.99 > 0.99 > 0.99 > 0.99 > 0.99
Efficiency (%) 97 98 103 96.4 88 100
LOD95% (copies/μl) 2.6 5.7 27 716 215 47
Detection rate limit (100%, copies/μl) 10 10 100 1000 1000 100
Ct cut-off 38 39 34 32 34 34
  1. LOD95%, limit of detection 95%; Ct, cycle threshold