Fig. 5

RIP-Sequencing of RNAs bound to the EBNA1 protein reveal that EBNA1 is bound to cellular RNAs in cellulo; (a) Statistical filtering used to select only high confidence peaks from the RIPSeeker analysis. To be selected, peaks needed to pass the following thresholds: Adjusted Pvalue lower than 0.05; False discovery rate lower than 10%; count in the EBNA1 analysis higher than the control; fold-enrichment higher than 1.5; manual curation do not show a false-positive peak. This allowed us to select only 50 peaks (black slice) from the 503 outputted by the analysis, from which one of the peaks was separated into two distinct peaks upon manual curation, for a total of 51; (b) Distribution of annotations corresponding to identified peaks from the ENSEMBL database. Annotations were retrieved using the online BioMart interface; (c) Gene ontology analysis for biological processes, cellular compartments and molecular functions of the 51 annotations retrieved from selected RIPSeeker peaks. The DAVID Bioinformatics Resources version 6.8 was used to retrieve enrichment, using the human genome as a control. A statistical threshold of less than 0.05 on the Benjamini corrected Pvalue was used