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Fig. 4 | Virology Journal

Fig. 4

From: Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells

Fig. 4

Progression of HIV-infected p24+CycT1- cells into latency following activation of memory CD4 T cells. Memory CD4 T cells were purified from peripheral blood, then uninfected or infected with HIV (R5 strain SF162) in IL2 medium for 2 days. Cell were washed and cultured with CD3+CD28 mabs and IL2 for 6 days. Cells were then stained for CycT1 and intracellular p24 in conjunction with either CD69/CD25, HLA.DR/CD38, or Ki67. a Shown are sample dotplots and (b) mean ± sem p24+CycT1- and p24+CycT1+ cells gated on CD25/CD69, HLA.DR/CD38, and Ki67 populations of HIV-infected memory CD4 T cells (*p < 0.05, N = 3–4)

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