Fig. 2

DENV-2 RNA level reduction in infected Vero cells after treatment with geraniin. The DENV-2 RNA production levels in infected Vero cells were quantified by SYBR Green dye-based qRT-PCR. a Standard curves generated using serial 10-fold dilutions of stock DENV-2 RNA with known amount of virus. The amplification efficiencies are 109 and 102% for the targeted E gene and the reference gene, β-actin respectively. b A complete inhibition of DENV-2 intracellular RNA production was observed after treatment with 26.26–105.04 μM geraniin. c A dose-response curve showing concentration-dependent inhibition of DENV-2 RNA production when geraniin was simultaneously added with DENV-2 during adsorption. Data are shown as the mean ± SEM of triplicate from one independent experiment