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Fig. 7 | Virology Journal

Fig. 7

From: Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks

Fig. 7

Construction of chimeric cDNA between pPVS-H-FL-β and pPVS-H-FL-H, and the infectivity of RNA transcripts. A unique BsiWI restriction site was introduced by C5850G substitution without an amino acid change by PCR. Two chimeric cDNA clones between pPVS-H-FL-β and pPVS-H-FL-H were constructed by exchanging ORF1 sequence at the BsiWI restriction site. The length of each poly(A) tail was determined by DNA sequence analysis. Capped RNA transcribed from each clone was used to inoculate N. occidentalis plants

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