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Table 1 Amino acid variations identified in the F50, F91 and F120 passages of PRV JS-2012

From: Comparative genomic analyses of a virulent pseudorabies virus and a series of its in vitro passaged strains

Gene Amino acid variations found in F50, F91, and F120 compared to JS-2012a
F50 F91 F120
UL6 P542S, P553S P542S, P553S P542S, P553S
UL8 S542A S542A S542A
UL10 (gM) V256A V256A V256A
UL15 G169E, P669D G169E, P669D G169E, P669D
UL16 ND 317(+LPRH), S322P, 325–327(IPE > NKR), 329–330(IN>LK), 331–332(DY>) 317(+LPRH), S322P, 325–327(IPE > NKR), 329–330(IN>LK), 331–332(DY>)
UL17 G237D, A241S, T249A, P253, A255P, D258A, 259(+GGG), N263D, P273L, P374L G237D, A241S, T249A, P253, A255P, D258A, 259(+GGG), N263D, P273L, P374L G237D, A241S, T249A, P253, A255P, D258A, 259(+GGG), N263D, P273L, P374L
UL18 (VP23) H47Y, P60A, H69Q, S79G, A142V, T270 M H47D, P60A, S79G, A142G, T270 M H47D, P60A, S79G, A142G, T270 M
UL19 (VP5) I178M, I1315T I178M I178M
UL22 (gH) P433L, A618V P433L, A618V P433L, A618V
UL25 L23P L23P L23P
UL26 (VP24) M124 T, I125L, R131L, S132C, Q136R, S137R, R139G, L143 V, T146A, V153A, Q161R M124 T, I125L, R131L, S132C, Q136R, S137 V, R139G, L143 V, T146A, V153A, Q161R, A455 M124 T, I125L, R131L, S132C, Q136R, S137R, R139G, L143 V, T146A, V153A, Q161R
UL26.5 ND A216 ND
UL28 (ICP18.5) A413P, D414E, D425G, 425(+GA), V430G, D432G, A522V A413P, D414E, D425G, V430G, D432G, A522V A413P, D414E, D425G, 425(+GA), V430G, D432G, A522V
UL33 P39A P39A P39A
UL34 A177V, T178S A177V, T178S A177V, T178S
UL36 (VP1/2) T2832A T2832A T2832A
UL37 E240D, F629 L, G762R E240D, F629 L, G762R E240D, F629 L, G762R
UL38 (VP19c) A218V A218V A218V
UL40 (RR2) A176T A176T A176T
UL44 (gC) R107H G90D, R107H G90D, R107H
UL46 (VP11/12) ND ND 599–626(PLTRHGSMRTSFRRGVRAAQRFVRRRLS>), 629–631(SAE > TTT), A633P, 635–674(RASGDSASAAAPAAASARGETDHVYQHPRPRTRADDGLYQ>), Q675G, 678–695(PVIDLTGHRASRRKSWRV>)
UL48 (VP16) R39Q,P89A R39Q,P89A R39Q,P89A
UL49 (VP22) R168H, N198D R168H R168H
UL49.5 (gN) T87A T87A T87A
UL50 (dUTPase) S209A S209A G191R,S209A
UL53 (gK) P164L, P171L P164L, P171L P164L, P171L
UL54 (ICP27) W20R, C48R, S156F,Q182R W20R, C48R, S156F,Q182R W20R, C48R,S156F,Q182R
US8 (gE), US9, US2 Deletion Deletion Deletion
IE180 (ICP4) P468S, G1385R S187 L, P468S, G1385R L76P, P468S, G1385R
  1. ND referred to no difference
  2. aSingle amino acid residues changes were recored in the following format, including the JS-2012 reference strain amino acid, its position, and the amino acid residue found in the passaged strains. Insertions were indicated by the amino acid position in JS-2012 followed by “+” and the new amino acid in passaged strains. Deletions were indicated by the symbol . Sequential changes are shown with the JS-2012 amino acid positions first, followed by the relevant JS-2012 amino acid residues, then with “>”, and finally the alternative amino aicd residues of passaged strains