Comparative genomic analyses of a virulent pseudorabies virus and a series of its in vitro passaged strains

Ye, Chao; Wu, Jiqiang; Tong, Wu; Shan, Tongling; Cheng, Xuefei; Xu, Jingjing; Liang, Chao; Zheng, Hao; Li, Guoxin; Tong, Guangzhi

doi:10.1186/s12985-018-1102-8

Virology Journal

Table 1 Amino acid variations identified in the F50, F91 and F120 passages of PRV JS-2012

From: Comparative genomic analyses of a virulent pseudorabies virus and a series of its in vitro passaged strains

Gene	Amino acid variations found in F50, F91, and F120 compared to JS-2012^a
Gene	F50	F91	F120
UL6	P542S, P553S	P542S, P553S	P542S, P553S
UL8	S542A	S542A	S542A
UL10 (gM)	V256A	V256A	V256A
UL15	G169E, P669D	G169E, P669D	G169E, P669D
UL16	ND	317(+LPRH), S322P, 325–327(IPE > NKR), 329–330(IN>LK), 331–332(DY>△)	317(+LPRH), S322P, 325–327(IPE > NKR), 329–330(IN>LK), 331–332(DY>△)
UL17	G237D, A241S, T249A, P253△, A255P, D258A, 259(+GGG), N263D, P273L, P374L	G237D, A241S, T249A, P253△, A255P, D258A, 259(+GGG), N263D, P273L, P374L	G237D, A241S, T249A, P253△, A255P, D258A, 259(+GGG), N263D, P273L, P374L
UL18 (VP23)	H47Y, P60A, H69Q, S79G, A142V, T270 M	H47D, P60A, S79G, A142G, T270 M	H47D, P60A, S79G, A142G, T270 M
UL19 (VP5)	I178M, I1315T	I178M	I178M
UL22 (gH)	P433L, A618V	P433L, A618V	P433L, A618V
UL25	L23P	L23P	L23P
UL26 (VP24)	M124 T, I125L, R131L, S132C, Q136R, S137R, R139G, L143 V, T146A, V153A, Q161R	M124 T, I125L, R131L, S132C, Q136R, S137 V, R139G, L143 V, T146A, V153A, Q161R, A455△	M124 T, I125L, R131L, S132C, Q136R, S137R, R139G, L143 V, T146A, V153A, Q161R
UL26.5	ND	A216△	ND
UL28 (ICP18.5)	A413P, D414E, D425G, 425(+GA), V430G, D432G, A522V	A413P, D414E, D425G, V430G, D432G, A522V	A413P, D414E, D425G, 425(+GA), V430G, D432G, A522V
UL33	P39A	P39A	P39A
UL34	A177V, T178S	A177V, T178S	A177V, T178S
UL36 (VP1/2)	T2832A	T2832A	T2832A
UL37	E240D, F629 L, G762R	E240D, F629 L, G762R	E240D, F629 L, G762R
UL38 (VP19c)	A218V	A218V	A218V
UL40 (RR2)	A176T	A176T	A176T
UL44 (gC)	R107H	G90D, R107H	G90D, R107H
UL46 (VP11/12)	ND	ND	599–626(PLTRHGSMRTSFRRGVRAAQRFVRRRLS>△), 629–631(SAE > TTT), A633P, 635–674(RASGDSASAAAPAAASARGETDHVYQHPRPRTRADDGLYQ>△), Q675G, 678–695(PVIDLTGHRASRRKSWRV>△)
UL48 (VP16)	R39Q,P89A	R39Q,P89A	R39Q,P89A
UL49 (VP22)	R168H, N198D	R168H	R168H
UL49.5 (gN)	T87A	T87A	T87A
UL50 (dUTPase)	S209A	S209A	G191R,S209A
UL53 (gK)	P164L, P171L	P164L, P171L	P164L, P171L
UL54 (ICP27)	W20R, C48R, S156F,Q182R	W20R, C48R, S156F,Q182R	W20R, C48R,S156F,Q182R
US8 (gE), US9, US2	Deletion	Deletion	Deletion
IE180 (ICP4)	P468S, G1385R	S187 L, P468S, G1385R	L76P, P468S, G1385R

ND referred to no difference
^aSingle amino acid residues changes were recored in the following format, including the JS-2012 reference strain amino acid, its position, and the amino acid residue found in the passaged strains. Insertions were indicated by the amino acid position in JS-2012 followed by “+” and the new amino acid in passaged strains. Deletions were indicated by the symbol △. Sequential changes are shown with the JS-2012 amino acid positions first, followed by the relevant JS-2012 amino acid residues, then with “>”, and finally the alternative amino aicd residues of passaged strains

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ISSN: 1743-422X

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