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Fig. 1 | Virology Journal

Fig. 1

From: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis

Fig. 1

Expression of the recombinant N protein. a The rN protein expressed in E. coli Unstained MW Marker (ThermoFisher Scientific); Lane 2: Recombinant E. coli strain BL21 star (DE3) before protein expression induction; Lane 3: Recombinant E. coli strain BL21 star (DE3) after protein expression induction; Lane 4: Purified rN protein. b Western blot characterization of the rN protein performed with MAb Anti6xHis. Lane 1: Spectra Multicolor Broad Range Protein Ladder (ThermoFisher Scientific); Lane 2: Recombinant N Protein; Lane 3: Negative control E. coli strain BL21 star (DE3)

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