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Fig. 1 | Virology Journal

Fig. 1

From: The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV production

Fig. 1

Downregulation of eEF1A levels by siRNA causes loss of actin stress fibers in uninfected and RSV infected HEp-2 cells. HEp-2 cells treated for 48 h with siRNA were (a) left uninfected or (b) infected with RSV. After 96 h p.t., the infected cells were fixed and stained with (a and b) Alexa Fluor 647 phalloidin and (b, second row) an anti-RSV polyclonal antibody, and counterstained with an appropriate secondary antibody conjugated to FITC. (b, third row) A merged image showing actin and RSV staining. Cells were imaged by confocal microscopy and a maximum intensity projection is shown. Examples of stress fibers in uninfected (a) and infected cells (b) are indicated by arrows

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