Fig. 1From: The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV productionDownregulation of eEF1A levels by siRNA causes loss of actin stress fibers in uninfected and RSV infected HEp-2 cells. HEp-2 cells treated for 48 h with siRNA were (a) left uninfected or (b) infected with RSV. After 96 h p.t., the infected cells were fixed and stained with (a and b) Alexa Fluor 647 phalloidin and (b, second row) an anti-RSV polyclonal antibody, and counterstained with an appropriate secondary antibody conjugated to FITC. (b, third row) A merged image showing actin and RSV staining. Cells were imaged by confocal microscopy and a maximum intensity projection is shown. Examples of stress fibers in uninfected (a) and infected cells (b) are indicated by arrowsBack to article page