Fig. 7
From: Porcine epidemic diarrhea virus S1 protein is the critical inducer of apoptosis
PEDV CV777 and new-emerging strain BJ2011 S1 protein Could also induce apoptosis in VERO-E6 even in BHK-21 cells. pEGFP-N1 and the recombinant plasmids expressing CV777 or BJ2011 S1 protein were transfected into confluent Vero-E6 or BHK-21 monolayer cells respectively. 48 h later, the cells were harvested and analyzed by Fluorescence-activated cells sorter (FACS), GFP-positive cells were gated for apoptosis analysis. (a) PEDV CV777 and new-emerging strain BJ2011 S1 protein Could also induce apoptosis in Vero-E6 even in BHK-21 cells. (b) Percentages of annexin-V-PE positive Vero-E6 Cells from gated cells are shown with graphs. Results are representative of three independent experiments. (c) Percentages of annexin-V-PE positive BHK-21 Cells from gated cells are shown with graphs. Results are representative of three independent experiments. (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, no significant)