Fig. 1

Expression and purification of recombinant RVFV-N protein. Recombinant plasmid containing the full length RVFV N gene was transformed into E. coli XL1-blue strain and induced with IPTG. E.coli cells were collected and dissolved in 10 mM PBS (pH 7.5) with 500 mM NaCl. After sonication, the E. coli cell lysate was centrifuged and the recombinant protein was purified from the supernatant by Talon™ IMAC affinity column. The E. coli cell lysate and purified recombinant protein were analyzed in a 10% SDS-PAGE gel and revealed with Coomassie brilliant blue staining. Lane 1: protein marker (Precision plus protein standards, Bio-Rad); Lane 2: supernatant of sonicated E. coli cell lysate after centrifugation; Lane 3: pellet of sonicated E.coli cell lysate; Lane 4: purified recombinant protein