Fig. 2

Resting T cells stimulated by LEC can be productively infected while remaining in resting state. Resting T cells were cultured alone or with human lymphatic endothelial cells (LEC). + and - indicate treatment with or without IFN-γ respectively in LEC. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture. On day 6 post-infection, T cells were stained to test for the presence of three activation markers: CD25-PE, CD69-APC, and DR-PerCP. Levels of activation are shown in (a) and levels of infection (%GFP+) are shown in (b). Samples were taken in triplicates and means +/− standard errors are plotted. Data shown are representative of three independent experiments yielding similar results. c Mean fluorescence intensity of the GFP+ cells, measured on day 6 post-infection, is shown. Samples were taken in triplicates and means +/− standard errors are plotted. Data shown are representative of three independent experiments yielding similar results