Fig. 1

Kinetics of viral infection in resting CD4+ T cells co-cultured with LEC compared with HUVEC. Resting T cells were cultured alone, with human umbilical vein endothelial cells (EC), or human lymphatic endothelial cells (LEC). + and - indicate treatment with or without IFN-γ respectively in EC or LEC. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture, and the %GFP+ cells were measured a on days 3, 6, and 8 post-infection. Infection rates were substantially higher in LEC-stimulated resting T cells than in resting T cells alone (statistically significant, Student’s T-tests, p values 1.6 × 10^− 6, 1.0 × 10^− 7, and 7.8 × 10^− 5 on days 3, 6, and 8, respectively) b A comparison of infection levels in LEC+ and LEC- stimulated T cells on day 6 and 8 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of seven independent experiments yielding similar results. c Mean fluorescence intensity data for GFP+ cells taken on day 8 post-infection. Samples were taken in triplicates and means+/− standard errors are plotted. Data shown are representative of seven independent experiments yielding similar results