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Table 2 Inter-assay of the mOTNRT-PCR

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

 

Target

Conc. (copies/reaction)

Number of determinations

Mean Ct

SD

CV(%)

Inter-assay

RSV

5 × 106

3

14.19

0.52

3.67

5 × 104

3

20.49

0.65

3.19

5 × 102

3

26.86

0.22

0.83

HRV

5 × 106

3

14.21

0.27

1.87

5 × 104

3

21.38

0.41

1.91

5 × 102

3

28.04

0.60

2.13

HMPV

5 × 106

3

12.34

0.43

3.45

5 × 104

3

19.29

0.37

1.91

5 × 102

3

27.75

0.33

1.20