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Table 2 Inter-assay of the mOTNRT-PCR

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

  Target Conc. (copies/reaction) Number of determinations Mean Ct SD CV(%)
Inter-assay RSV 5 × 106 3 14.19 0.52 3.67
5 × 104 3 20.49 0.65 3.19
5 × 102 3 26.86 0.22 0.83
HRV 5 × 106 3 14.21 0.27 1.87
5 × 104 3 21.38 0.41 1.91
5 × 102 3 28.04 0.60 2.13
HMPV 5 × 106 3 12.34 0.43 3.45
5 × 104 3 19.29 0.37 1.91
5 × 102 3 27.75 0.33 1.20