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Fig. 1 | Virology Journal

Fig. 1

From: A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus

Fig. 1

The sensitivity analysis and standard curves of the mOTNRT-PCR assay. a, b, and c are the amplification curves of RSV, HRV and HMPV, respectively using serial 10-fold dilutions of the mixed recombinant plasmids from 108 to 100 copies/μL, and d is the standard curves of the mOTNRT-PCR assay. The R2 of RSV, HRV and HMPV were 0.997, 0.994, 0.995 and the E of RSV, HRV and HMPV were 98.5, 95.7 and 92.5%, respectively

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