Fig. 1

Evaluation of in vitro envelope (E) glycoprotein expression by immunofluorescence assay (IFA) and western blot analysis. a DEF cells were transfected with pSCA1-E or the negative control plasmid pSCA1. At 48 h post-transfection, cells were stained using rabbit anti-E polyclonal antiserum and a FITC-conjugated goat anti-rabbit IgG, and observed with a fluorescence microscope. b Western blot analysis of E glycoprotein expression. Lane 1, Pre-stained protein marker; lane 2, lysate generated from DEF cells transfected with pSCA1-E plasmid; lane 3, lysate generated from DEF cells transfected with empty pSCA1 vector