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Fig. 2 | Virology Journal

Fig. 2

From: A loop-mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathy

Fig. 2

Cross-reactivity of JCV in the LAMP assay for JCV genome detection. a Agarose gel electrophoresis of the LAMP products performed with the polyomavirus DNAs (JCV, BKV, SV40, and MPyV) and no template control (NTC). b The LAMP-positive product (lane 1) and its SspI digested product (lane 2). c Visual detection of the LAMP products by adding nucleic acid staining reagent under the transilluminator. d The LAMP reaction was performed with viral DNA (HSV-1, VZV, and HIV type 1 subtype B and C) or RNA (JEV, WNV, LCMV, MeV, and RV) and an NTC. JCV, JC polyomavirus; BKV, BK virus; SV40, simian virus 40; MPyV, murine polyomavirus; HSV-1, herpes simplex virus type 1; VZV, varicella-zoster virus; HIV, human immunodeficiency virus; JEV, Japanese encephalitis virus; WNV, West Nile virus; LCMV, lymphocytic choriomeningitis virus; MeV, measles virus; RV, rabies virus

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Virology Journal

ISSN: 1743-422X