Fig. 1

UBE2J1 is involved in DENV infection. a RNAi screening of UBE2 family during DENV infection: HEK293T cells were transfected with siRNAs for each specific gene of UBE2 family, and infected with DENV for 48 h at an MOI = 1. The viral loads were analyzed by measuring the DENV E gene copy using qRT-PCR and normalized to a human β-actin gene. The relative replication level of DENV in nonsense siRNA (N.C.) transfected cells was set to 1. The red lines were set to mark the significant suppression (fold change < 0.5) or stimulation (fold change > 1.5) of DENV RNA replication. Results were expressed as the mean + SD. Each siRNA treatment was compared to N.C. group individually: * p < 0.05 and ** p < 0.01 (t-test). b The qRT-PCR analysis of UBE2J1 mRNA expression in A549, PBMCs, and HEK293T before and after DENV infection. Results were expressed as the mean + SD. * p < 0.05 and ** p < 0.01 (t-test). (C) Immunoblots analysis of UBE2J1 expression in HEK293T cells before and after DENV infection. The relative grey density of immunoblots bands (UBE2J1/β-actin) was analyzed by ImageJ software. Representative results from at least 3 independent experiments