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Fig. 3 | Virology Journal

Fig. 3

From: Localization of cassava brown streak virus in Nicotiana rustica and cassava Manihot esculenta (Crantz) using RNAscope® in situ hybridization

Fig. 3

Localization of CBSV-Mo83 RNA in cross-sections of Nicotiana rustica stem tissues. A Acquisitions using light microscopy. Upper images: tissue from healthy control plants. Lower images: tissue from plants infected with CBSV-Mo83. Abundant presence of viral RNA in cells is visualized as single red dots or dots merged into signal clusters (arrows). B Acquisition by confocal laser scanning microscopy. Upper images: non-infected control. Lower images: CBSV-Mo83-infected section. The Fast-Red signal (left) laid over the image from transmitted light (centre) results in a merged picture (right) to demonstrate virus detection in infected cells. Pi, pith; Xy, xylem; Ph, phloem

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