Fig. 3

The effect of quercetin on EV71 replication in RD cells. RD cells infected with 100 TCID₅₀ of EV71 were incubated in the absence or presence of 50 μM quercetin and harvested at the indicated times pi. a The progeny viral yields were determined. b Total RNA was extracted from cells and culture supernatants and EV71 RNA levels were measured. Cellular actin amplification was used for normalization. c EV71-protein was determined by indirect immunofluorescence using a mouse anti-enterovirus 71 monoclonal antibody and an Alexa-Fluor-488-conjugated AffiniPure goat anti-mouse IgG (H + L). The nucleus was stained with DAPI, the green foci indicate the presence of EV71 protein. Values represent the means ± SDs of three independent experiments. *P < 0.05; **P < 0.01, compared with EV71 control group