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Fig. 1 | Virology Journal

Fig. 1

From: Trigger factor assisted self-assembly of canine parvovirus VP2 protein into virus-like particles in Escherichia coli with high immunogenicity

Fig. 1

Expression and purification of CPV VP2 protein co-expressed with Tf16. CPV VP2 protein were analyzed by 12% SDS-PAGE and Western blot using an anti-CPV polyclonal antibody a SDS-PAGE analysis of the VP2 protein expression. Lane M, protein molecular weight (MW) markers; Lane 1, pET30a vector control; Lane 2, pET30a-VP2 cells before induction of IPTG; Lane 3, induced pET30a-VP2 cells; Lane 4, induced pET30a-VP2 cell lysate; Lane 5, inclusion body of induced pET30a-VP2 cells; Lane 6, pET30a-VP2-Tf16 cells before induction of IPTG and L-Arabinose; Lane 7, induced pET30a-VP2-Tf16 cells; Lane 8, induced pET30a-VP2-Tf16 cell lysate; Lane 9, inclusion body of induced pET30a-VP2-Tf16 cells. b SDS-PAGE analysis of purification of VP2 protein co-expressed with chaperone Tf16. Lane 1, cells before induction; Lane 2, induced cells lysate; Lane 3, flow buffer; Lane 4, wash buffer; Lane 5, purified VP2 protein. c Western-blot analysis of purified VP2 protein with an anti-CPV polyclonal antibody. Lane1, purified VP2 protein; Lane 2, induced cell lysate; Lane 3, cells before induction. The MW of VP2 protein is approximately 70 kDa. The Tf16 is approximately 56 kDa

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