Table 1 Primers used for RT-PCR
Gene or bacterial target | Primer sequence (5′-3′) | Product size |
|---|---|---|
GAPDH | F: TCATCATCTCTGCCCCTTCT | 172 bp |
R: GTCATGAGTCCCTCCACGAT | ||
IL-1β | F:AGAGGGACATGGAGAAGCGA | 209 bp |
R: GCCCTCTGGGTATGGCTTT | ||
IL-6 | F:CCTCGGCAAAATCTCTGCAA | 189 bp |
R: TGAAACTCCACAAGACCGGT | ||
IL-8 | F: CCTCATTCCTGTGCTGGTCA | 273 bp |
R:TGCAAGTTGAGGCAAGAAGAC | ||
TGF-β | F: CGCGTGCTAATGGTGGAAAG | 132 bp |
R: TGCCCGAGAGAGCAATACAG | ||
TNF-α | F:GCCCTTCCACCAACGTTTTC | 158 bp |
R: TCCCAGGTAGATGGGTTCGT | ||
IL-10 | F: TCTGAGAACAGCTGCATCCAC | 112 bp |
R: CGCCCATCTGGTCCTTCGTT | ||
Total bacteria | F: GTGSTGCAYGGYYGTCGTCA | 147 bp |
R: ACGTCRTCCMCNCCTTCCTC | ||
Lactobacillus | F: AGAGGTAGTAACTGGCCTTTA | 391 bp |
R: GCGGAAACCTCCCAACA | ||
Entero-bacteriaceae | F: ATGGCTGTCGTCAGCTCGT | 385 bp |
R: CCTACTTCTTTTGCAACCCACTC |