Skip to main content

Table 2 Cycle threshold (Ct) values obtained for one HBeAg-positive sample treated (or not treated) with DNase or RNase after nucleic acid extraction

From: High serum levels of pregenomic RNA reflect frequently failing reverse transcription in hepatitis B virus particles

  DNA amplification (PCR with DNA master mix and no RT step)
Ct value
RNA amplification (RT-PCR with RNA master mix (incl reverse transcriptase and an initial RT step)
Ct value
No enzymatic pretreatment 18.49 17.63
DNase 30.76 19.26
RNase 18.98 18.52
DNase and RNase 33.56 30.85