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Table 2 Cycle threshold (Ct) values obtained for one HBeAg-positive sample treated (or not treated) with DNase or RNase after nucleic acid extraction

From: High serum levels of pregenomic RNA reflect frequently failing reverse transcription in hepatitis B virus particles

 

DNA amplification (PCR with DNA master mix and no RT step)

Ct value

RNA amplification (RT-PCR with RNA master mix (incl reverse transcriptase and an initial RT step)

Ct value

No enzymatic pretreatment

18.49

17.63

DNase

30.76

19.26

RNase

18.98

18.52

DNase and RNase

33.56

30.85

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Virology Journal

ISSN: 1743-422X