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Table 4 Spot intensities of the peroxiredoxin proteins

From: HIV-1 tat expression and sulphamethoxazole hydroxylamine mediated oxidative stress alter the disulfide proteome in Jurkat T cells

SMX-HA 0μM 200μM
Dox (ng/ml) 0 400 1000 0 400 1000
Jurkat E6.1 100 ± 1 n/d n/d 33 ± 1.4 n/d 37 ± 2.0
Jurkat HIV 44 ± 2.2*** n/d n/d 33 ± 0.6 n/d n/d
Tat101 31 ± 1.1*** 46 ± 1.4*** 35 ± 0.1*** 31 ± 4.4 8.5 ± 0.3***††† 23 ± 0.5***
Tat101GFP 34 ± 1.4*** 30 ± 0.6*** 32 ± 0.8*** 12 ± 2.4***††† 41 ± 1.4 18 ± 2.5***
Tat72GFP 44 ± 4.1*** 41 ± 0.3*** 39 ± 1.0*** 7.8 ± 0.1***††† 6.7 ± 1.2***††† 7.8 ± 0.7***†††
TatΔGFP 33 ± 4.0*** 43 ± 0.6*** 35 ± 0.6*** 13 ± 1.1** 10 ± 0.1*** 9.5 ± 0.4***
  1. Values for each of the R2D gels were calculated as a percentage of the spot intensity for protein 1 of the Jurkat E6.1 sample. n/d: not done. ***p < 0.001, **p < 0.01 vs. Jurkat E6.1 and †††p < 0.001, p < 0.05 vs. Jurkat HIV