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Table 4 Spot intensities of the peroxiredoxin proteins

From: HIV-1 tat expression and sulphamethoxazole hydroxylamine mediated oxidative stress alter the disulfide proteome in Jurkat T cells

SMX-HA

0μM

200μM

Dox (ng/ml)

0

400

1000

0

400

1000

Jurkat E6.1

100 ± 1

n/d

n/d

33 ± 1.4

n/d

37 ± 2.0

Jurkat HIV

44 ± 2.2***

n/d

n/d

33 ± 0.6

n/d

n/d

Tat101

31 ± 1.1***†

46 ± 1.4***

35 ± 0.1***

31 ± 4.4

8.5 ± 0.3***†††

23 ± 0.5***

Tat101GFP

34 ± 1.4***

30 ± 0.6***†

32 ± 0.8***

12 ± 2.4***†††

41 ± 1.4

18 ± 2.5***†

Tat72GFP

44 ± 4.1***

41 ± 0.3***

39 ± 1.0***

7.8 ± 0.1***†††

6.7 ± 1.2***†††

7.8 ± 0.7***†††

TatΔGFP

33 ± 4.0***

43 ± 0.6***

35 ± 0.6***

13 ± 1.1**†

10 ± 0.1***†

9.5 ± 0.4***†

  1. Values for each of the R2D gels were calculated as a percentage of the spot intensity for protein 1 of the Jurkat E6.1 sample. n/d: not done. ***p < 0.001, **p < 0.01 vs. Jurkat E6.1 and †††p < 0.001, †p < 0.05 vs. Jurkat HIV
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Virology Journal

ISSN: 1743-422X