Fig. 1

Representative western blot and slot blot data showing SMX-HA-induced haptenation. a Western blot of cells treated with 0 (lanes 1–3), 100 μM (lanes 4–6) and 200 μM (lanes 7–9) SMX-HA for 2 h and resolved on 12% SDS-PAGE. Lanes 1, 4, 7: Jurkat E6.1; lanes 2, 5, 8: Tat101GFP; lanes 3, 6, 9: Tat72GFP. b Total protein from cells treated with various concentrations of SMX-HA and applied onto a membrane via a slot blot apparatus. Both membranes were blotted with anti-SMX-HA antibody. The band intensities were measured digitally and the ratio of SMX-HA haptenation to GAPDH content in the corresponding sample was calculated. (C and D) The SMX-HA/GAPDH ratio increases with increasing concentration of SMX-HA for GFP (c) and wildtype Tat101 (d) expressing Jurkat E6.1 cells. Significance was defined as follows: a, c, e P < 0.05 vs. 0, 400, 1000 ng Dox ml^− 1 at 0 μM SMX-HA, respectively. b, d, f P < 0.05 vs. 0, 400, 1000 ng Dox ml^− 1 at 50 μM SMX-HA, respectively. g P < 0.05 vs. 200 ng Dox ml^− 1 at 100 μM SMX-HA. e Expression of wildtype Tat101 and Tat mutants does not result in significantly different SMX-HA/GAPDH ratios compared to untransfected Jurkat E6.1 and GFP expressing cells. Data are expressed as mean of three independent experiments