Fig. 4

Recombinant CedPV induces an IFN response. HeLa-CCL2 cells were infected with medium with no virus (mock), infected with rCedPV-wt (MOI: 0.5, 1.0, 5.0) or transfected with Poly I:C (1 μg/mL). Twenty-four hours post-infection, total RNA was extracted, of which 500 ng was used for q-RT-PCR for IFN-α, IFN-β and 18S ribosomal RNA. Each sample was analyzed in triplicate and fold changes were calculated relative to 18S ribosomal RNA and normalized to mock samples using the ΔΔCt method. The graphs are representative of two independent experiments performed in triplicate. Error bars indicate standard deviation.* p < 0.05 and ** p < 0.01