Fig. 7

Complementation of the viral polymerase activity by NS1. CLEC213 cells in 24-well plates were transfected with the plasmid set of the pHW-based H7-minireplicon system (see Methods section), along with an NS1-expression vector or an empty vector control. Twenty-four hours later, cells were lyzed and the Firefly-luciferase activity of the lysates, resulting from translation of the polymerase-transcribed mRNA, was measured and normalized relative to the Renilla-luciferase through a dual luciferase assay. Each point represents the normalized polymerase activity (geometric mean of a technical triplicate) relative to the empty-vector control in a given experiment (numbers are the mean and s.e.m from three independent experiments). The “no-PB1” control values (not shown) were less than 0.1%. * P < 0.05; *** P < 0.001; ****P < 0.0001 in a Dunnett’s test comparing each set to the control (empty pCI) condition