Fig. 5
From: Inhibition of HIV early replication by the p53 and its downstream gene p21
The analysis of HIV-1 infection after siRNA knockdown of p53 and p21 in non-cycling HCT116 p53+/+ cells. a Western blot of p53, p21, RNR2, SAMHD1 and pSAMHD1(T-592) proteins in non-cycling HCT116 p53+/+ cells after siRNA transfection. b Quantification of percentage of p21 after siRNA knockdown of p21 and siRNA knockdown of p53. c Quantification of percentage of p53 after siRNA knockdown of p21 and siRNA knockdown of p53. d Cell cycle analysis after siRNA transfections by Click-iT™ Plus EdU Flow Cytometry Assay. e Quantification of infection in non-cycling HCT116 p53+/+ cells after siRNA knockdown of p21 and siRNA knockdown of p53. f Quantification HIV-1 RT in non-cycling HCT116 p53+/+ cells after siRNA knockdown of p21 and siRNA knockdown of p53. 2.0 MOI HIV-1 was used to infect non-cycling HCT116 p53+/+ cells in the quantification of infection and late RT. The non-targeting (NT) siRNA was used as negative control. HIV-1 RT was quantified by TaqMan real time PCR at 16 h post infection. The results represented a triplicate experiment. In Student’s t-test, p value < 0.05 is indicated by *