Molecular analysis of partial VP-2 gene amplified from rectal swab samples of diarrheic dogs in Pakistan confirms the circulation of canine parvovirus genetic variant CPV-2a and detects sequences of feline panleukopenia virus (FPV)

Ahmed, Nisar; Riaz, Adeel; Zubair, Zahra; Saqib, Muhammad; Ijaz, Sehrish; Nawaz-Ul-Rehman, Muhammad Shah; Al-Qahtani, Ahmed; Mubin, Muhammad

doi:10.1186/s12985-018-0958-y

Virology Journal

Table 1 Details of samples sequenced, place and location, year of collection, gene bank accessions and mutations at various amino acid residues on partial VP2 protein of Canine parvovirus detected from dogs. The positions of mutations is based on CPV-2 GenBank accession number M38245, AY742953

From: Molecular analysis of partial VP-2 gene amplified from rectal swab samples of diarrheic dogs in Pakistan confirms the circulation of canine parvovirus genetic variant CPV-2a and detects sequences of feline panleukopenia virus (FPV)

S. No	Sample No/ Year of collection	Gene bank Accession numbers	Place of collection	CPV genome nucleotide/amino acid positions and mutations				CPV type
S. No	Sample No/ Year of collection	Gene bank Accession numbers	Place of collection	3675/297 (T to G)	3756–3757/324 (TA to AT)	4062–4064/426 (AAT to GAT/GAA)	4105–4106/440 (AC to GC)	CPV type
1	01CPV2a15/2014	MF182906	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
2	04CPV2a15/2014	MF182907	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
3	05CPV2a15/2014	MF182908	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
4	06CPV2a15/2014	MF182909	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
5	07CPV2a15/2014	MF182910	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
6	09CPV2a15/2014	MF182911	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
7	13CPV2a15/2014	MF182912	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
8	14CPV2a15/2014	MF182913	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
9	15CPV2a15/2014	MF182914	Faisalabad	G	AT	GAT	AC	New2a (Ser297 → Ala)
10	16CPV2a15/2015	MF182915	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
11	17CPV2a15/2015	MF182916	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
12	18CPV2a15/2015	MF182917	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
13	19CPV2a15/2015	MF182918	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
14	20CPV2a15/2015	MF182919	Faisalabad	G	AT	GAT	AC	New2a (Ser297 → Ala)
15	21CPV2a15/2015	MF182920	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
16	23CPV2a15/2015	MF182921	Faisalabad	G	AT	GAT	AC	New2a (Ser297 → Ala)
17	24CPV2a15/2015	MF182922	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
18	25CPV2a15/2015	MF182923	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
19	26CPV2a15/2015	MF182924	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
20	28CPV2a15/2015	MF182925	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
21	32CPV2a15/2015	MF182926	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
22	33CPV2a15/2015	MF182927	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
23	34CPV2a15/2015	MF182928	Faisalabad	G	AT	GAT	GC	New2a (Ser297 → Ala)
24	38CPV2a15/2015	MF182929	Faisalabad	G	AT	GAT	AC	New2a (Ser297 → Ala)
25	39CPV2a15/2015	MF182930	Faisalabad	G	AT	GAT	AC	New2a (Ser297 → Ala)

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ISSN: 1743-422X

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