Fig. 2

The five MAbs specifically recognize the recombinant N protein of IYSV in Western blot. M, protein molecular weight marker; Lanes 1,3,5,7, and 9 were loaded with the protein extracts from the E. coli strain expressing the recombinant N protein, but the lanes 2,4,6,8, and10 were loaded with protein extracts from the E. coli strain expressing the empty vector. 1,2 were detected by 16D9; 3,4 were detected by 11C6; 5,6 were detected by 7F4; 7,l8 were detected by 12C10; 9,10 were detected by 14H12