Fig. 4

Relative luciferase activities in Huh7 S10–3 liver cells transfected with (a) RNA of HEV Rluc mutants containing Q1246H and V1213A in HEV genotype 3 Rluc replicons, and (b) RNA of A1213V mutation in genotype 4 Rluc replion. V1213A: mutation of V1213A; Q1246H: mutation of Q1246H; V1213A + Q1246H: mutation of Q1246H and V1213A; A1213V: mutation of A1213V. are measured at 5 days post-transfection and normalized with co-transfected firefly luciferase RNA. The error bars indicate standard deviations (SD). *, P < 0.05; **, 0.05 < P < 0.01; ***, P < 0.001