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Table 8 Performance of real-time RT-PCRs for M, H1av, H1hu, H1huΔ146–147, H1pdm, H3, N1, N1pdm and N2 on ten-fold dilutions of RNA extracted from swIAVs of different subtypes when run on thermocycler LightCycler®1536

From: Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

Virus strain Criteria M gene RT-qPCRa Real-time RT-PCR for molecular subtyping
H1av H1hu H1hu Δ146–147 H1pdm H3 N1 N1pdm N2
A/Sw/Cotes d’Armor/0388/2009 (H1avN1) Range of linearitya <12.01 to >35.45 <12.01 to 32.81      <12.01 to >35.45   
Slope −3.423 −3.386      −3.356   
R2 0.9963 0.9971      0.9914   
Efficacy 95.95% 97.38%      98.61%   
A/Sw/Cotes d’Armor/0186/2010 (H1avN2) Range of linearitya <15.01 to 32.76 <15.01 to 32.76        <15.01 to 32.76
Slope −3.5616 −3.2659        −3.674
R2 0.9992 0.9988        0.9962
Efficacy 90.89% 102.39%        87.15%
A/Sw/Cotes d’Armor/0113/2006 (H1huN2) Range of linearitya <14.52 to 28.49   <14.5 to 33.49       <14.52 to >33.49
Slope −3.637   −3.5244       −3.5025
R2 0.9888   0.9938       0.9866
Efficacy 88.34%   92.19%       92.98%
A/Sw/France/22–130212/2013 (H1huN2Δ146–147) Range of linearitya <8.59 to >32.73   <8.59 to >25.44 <8.59 to >32.73      <8.59 to >32.73
Slope −3.4642   −3.5856 −3.6888      −3.6624
R2 0.9989   0.9992 0.9979      0.9958
Efficacy 94.39%   90.06% 86.68%      87.52%
A/Sw/Cotes d’Armor/0070/2010 (H1huN1) Range of linearitya <14.85 to >29.68   <14.85 to 29.68     <14.85 to >29.68   
Slope −3.726   −3.307     −3.481   
R2 0.9952   0.9903     0.995   
Efficacy 85.52%   100.63%     93.76%   
A/Sw/Cotes d’Armor/0190/2006 (H1huN1Δ147) Range of linearitya <13.49 to 31.31   <13.49 to 25.01     <13.49 to >31.31   
Slope −3.6041   −3.724     −3.6323   
R2 0.9979   0.9958     0.9984   
Efficacy 89.44%   85.58%     88.50%   
A/Sw/Sarthe/0255/2010 (H1N1pdm) Range of linearitya <13.31 to 33.50     <13.31 to 31.88   <13.31 to 31.88 <13.31 to 31.88  
Slope −3.5216     −3.4394   −3.4324 −3.589  
R2 0.9927     0.9939   0.9887 0.9963  
Efficacy 92.29%     95.32%   95.59% 89.95%  
A/Sw/Flandres/1/1998 (H3N2) Range of linearitya <12.96 to >34.01      <12.96 to >34.01    <12.96 to 30.48
Slope −3.485      −3.5005    −3.8987
R2 0.9977      0.9931    0.9942
Efficacy 93.62%      93.05%    80.51%
  1. aThe range of linearity is given as the interval of Cq-values obtained from M gene RT-qPCR on diluted samples. R2 Coefficient of linear regression