Table 8 Performance of real-time RT-PCRs for M, H1_av, H1_hu, H1_{huΔ146–147}, H1pdm, H3, N1, N1pdm and N2 on ten-fold dilutions of RNA extracted from swIAVs of different subtypes when run on thermocycler LightCycler^®1536

A/Sw/Cotes d’Armor/0388/2009 (H1_avN1)

Range of linearity^a

<12.01 to >35.45

<12.01 to 32.81

<12.01 to >35.45

Slope

−3.423

−3.386

−3.356

R²

0.9963

0.9971

0.9914

Efficacy

95.95%

97.38%

98.61%

A/Sw/Cotes d’Armor/0186/2010 (H1_avN2)

Range of linearity^a

<15.01 to 32.76

<15.01 to 32.76

<15.01 to 32.76

Slope

−3.5616

−3.2659

−3.674

R²

0.9992

0.9988

0.9962

Efficacy

90.89%

102.39%

87.15%

A/Sw/Cotes d’Armor/0113/2006 (H1_huN2)

Range of linearity^a

<14.52 to 28.49

<14.5 to 33.49

<14.52 to >33.49

Slope

−3.637

−3.5244

−3.5025

R²

0.9888

0.9938

0.9866

Efficacy

88.34%

92.19%

92.98%

A/Sw/France/22–130212/2013 (H1_huN2_Δ146–147)

Range of linearity^a

<8.59 to >32.73

<8.59 to >25.44

<8.59 to >32.73

<8.59 to >32.73

Slope

−3.4642

−3.5856

−3.6888

−3.6624

R²

0.9989

0.9992

0.9979

0.9958

Efficacy

94.39%

90.06%

86.68%

87.52%

A/Sw/Cotes d’Armor/0070/2010 (H1_huN1)

Range of linearity^a

<14.85 to >29.68

<14.85 to 29.68

<14.85 to >29.68

Slope

−3.726

−3.307

−3.481

R²

0.9952

0.9903

0.995

Efficacy

85.52%

100.63%

93.76%

A/Sw/Cotes d’Armor/0190/2006 (H1_huN1_Δ147)

Range of linearity^a

<13.49 to 31.31

<13.49 to 25.01

<13.49 to >31.31

Slope

−3.6041

−3.724

−3.6323

R²

0.9979

0.9958

0.9984

Efficacy

89.44%

85.58%

88.50%

A/Sw/Sarthe/0255/2010 (H1N1pdm)

Range of linearity^a

<13.31 to 33.50

<13.31 to 31.88

<13.31 to 31.88

<13.31 to 31.88

Slope

−3.5216

−3.4394

−3.4324

−3.589

R²

0.9927

0.9939

0.9887

0.9963

Efficacy

92.29%

95.32%

95.59%

89.95%

A/Sw/Flandres/1/1998 (H3N2)

Range of linearity^a

<12.96 to >34.01

<12.96 to >34.01

<12.96 to 30.48

Slope

−3.485

−3.5005

−3.8987

R²

0.9977

0.9931

0.9942

Efficacy

93.62%

93.05%

80.51%