Fig. 3

Characterization of the CPZ effect. a-b. HepG2 and A549 cells were infected by EV71 at an MOI of 5 in the presence of CPZ (20 μM) or DMSO for 6 h, 12 h and 18 h. After removal of virus and the drug, cells were washed and continued to culture until 24 h and then processed for flow cytometry. c-d. HepG2 and A549 cells were infected by EV71 at an MOI of 5. CPZ (20 μM) or DMSO was then added at −2, 0, 2, 4, 6, 8, 10, 12 hpi. At 24 hpi, cells were collected for VP-1 staining. The infectivity was measured as percentage of VP-1 positive cells. e-f. HepG2 and A549 cells were infected with EV71 at an MOI of 5 for 12 h, and then CPZ (20 μM) or DMSO was added. Cells were harvested at different time points (12 h, 16 h, 18 h and 24 h) afterward and subjected to VP-1 staining. Results were presented as the mean with SD from three independent experiments. *, p < 0.05. CPZ, filled square; DMSO, filled circle