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Fig. 1 | Virology Journal

Fig. 1

From: Enhanced human enterovirus 71 infection by endocytosis inhibitors reveals multiple entry pathways by enterovirus causing hand-foot-and-mouth diseases

Fig. 1

The effect of CPZ on EV71 infection in HepG2 and A549 cells. a. HepG2 and A549 cells were pretreated with increasing concentrations of CPZ (10, 20, 30 and 40 μM) for 2 h at 37 °C before EV71 infection. At 24 hpi, the infected cells were processed for flow cytometry. The bar charts represented the EV71 infectivity determined by the percentage of VP-1 positive cells and were shown as means with SD from three independent experiments. *, p < 0.05. Cell viability upon CPZ treatments was shown by the curve above the chart. b-d. A549 cells were infected with EV71 at an MOI of 5 for 12 h and then CPZ or DMSO was added. At 24 hpi, cells were lysed, and eIF4G cleavage, EV71- 2C, 3C and VP-1 were determined by Western blot (b); EV71 VP-1 mRNA levels were measured by qRT-PCR and normalized to 18 s (c); Virus titers were determined and shown in TCID50 (d). The blot shown was representative of three independent experiments and the full-length bolts were presented in Additional file 1 Fig. S5. Results were presented as the mean with SD from three independent experiments. *, p < 0.05. UT, untreated cells; TCID50, 50% tissue culture infective dose

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