The CC chemokine ligand (CCL) 1, upregulated by the viral transactivator Tax, can be downregulated by minocycline: possible implications for long-term treatment of HTLV-1-associated myelopathy/tropical spastic paraparesis

Table 1 Primer sequences for reporter plasmid construction

Primer name	Direction	Sequence
-1541 CCL1p-F1	Forward	CAT CTC GAG TAT TTC ACC ACA TGT GCA TGG
-401 CCL1p-F2	Forward	CAT CTC GAG TGG CTG GTA TTC GTG GTG GTG
-281 CCL1p-F3	Forward	CAT CTC GAG GAA AAG TAA GTG AGA GGA AGT
-221 CCL1p-F4	Forward	CAT CTC GAG CAA GTA CTA CTG CTT CAG GGC
CCL1p-R	Reverse	CAT AAG CTT GGC TAA CCA AGG ACC TAG AAC

CTC GAG and AAG CTT (bold letters) are the respective restriction sites for XhoI and HindIII

ISSN: 1743-422X