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Table 1 Primer sequences for reporter plasmid construction

From: The CC chemokine ligand (CCL) 1, upregulated by the viral transactivator Tax, can be downregulated by minocycline: possible implications for long-term treatment of HTLV-1-associated myelopathy/tropical spastic paraparesis

Primer name Direction Sequence
-1541 CCL1p-F1 Forward CAT CTC GAG TAT TTC ACC ACA TGT GCA TGG
-401 CCL1p-F2 Forward CAT CTC GAG TGG CTG GTA TTC GTG GTG GTG
-281 CCL1p-F3 Forward CAT CTC GAG GAA AAG TAA GTG AGA GGA AGT
-221 CCL1p-F4 Forward CAT CTC GAG CAA GTA CTA CTG CTT CAG GGC
CCL1p-R Reverse CAT AAG CTT GGC TAA CCA AGG ACC TAG AAC
  1. CTC GAG and AAG CTT (bold letters) are the respective restriction sites for XhoI and HindIII